Collect cells by centrifugation for 5 minutes at 1100 rpm, remove supernatant and dilute cells to a concentration of 1x106 cells/ml in fresh pre-warmed medium. Immediately transfer your cells to the ...
In the limited space of the nucleus, most of the genome is tightly folded, leaving accessible only the parts that need to be transcribed. There is “huge interest” in determining which elements of the ...
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