What motivated you to develop the ACE technique? One of the limitations of mass cytometry is its low sensitivity. To reach the detection limit, the target needs multiple copies of the antibody tag ...

Flow cytometry, invented in the 1950s, uses antibodies linked to fluorescent probes to detect cell surface and intracellular proteins. Although able to achieve single-cell sensitivity, the method is ...

This article will review flow cytometry to help readers understand the challenges faced when increasing flow cytometer SNR (signal-to-noise ratio). Quick review: What is flow cytometry? Flow cytometry ...

Flow cytometry can be used to measure a number of characteristics of a population of cells and refers to the process of passing cells individually through a single or series of laser beams adjacent to ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...

Flow cytometry remains a critical technology for the high-throughput analysis of single cells in complex populations. Attention to good analysis practices is more important than ever due to the recent ...