STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to achieve an improvement in resolution, …

Aug 8, 2024 · In this Primer, we focus on the most recent advancements in stimulated emission depletion (STED) microscopy, encompassing optics, computational microscopy and probes …

In STED microscopy, the specimen is illuminated by two synchronized ultrafast co-linear sources consisting of an excitation laser pulse followed by a red-shifted depletion laser pulse that is …

Researchers have used STED to image the fine structures within neurons, including the distribution of synaptic proteins and the organization of synaptic vesicles, which are tiny sacs …

STED microscopy uses two laser pulses to localize fluorescence at each focal spot. The first pulse is used to excite a fluorophore to its fluorescent state, and the second pulse is a …

This interactive tutorial compares the stimulated emission depletion (STED) and stochastic optical reconstruction microscopy (STORM) imaging processes.

The STED donut determines where the fluorescence originates in space; therefore, imaging with a single STED line ensures that the different channels (colors) are intrinsically aligned.

Stimulated emission depletion microscopy (STED) is a fluorescence microscopy technique that overcomes the diffraction limited resolution of confocal microscopes.

STED, or Stimulated Emission Depletion microscopy, is a super-resolution imaging technique that allows scientists to visualize cellular structures at a resolution beyond the diffraction limit of light.

Jun 8, 2025 · In this series of articles on STED microscopy, I will cover more about how it works, optimising staining, and share some tips and tricks on how to get the most out of it.